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KMID : 0665220110240020258
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Korean Journal of Food and Nutrition
2011 Volume.24 No. 2 p.258 ~ p.267
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14-3-3-Associated Proteins in Helicobacter pylori-Infected Gastric Epithelial Cells
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Chung Hae-Yun
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Abstract
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14-3-3 is a highly conserved, ubiquitously expressed protein family. It associates with diverse cellular proteins through its specific phosphoserine/phosphothreonine-binding activity and thus contributes to the regulation of crucial cellular processes such as metabolism, signal transduction, cell-cycle control, apoptosis, protein trafficking, transcription and stress responses. This study aims to determine changes in levels of 14-3-3 isoforms and 14-3-3 - associated proteins in Helicobacter pylori(H. pylori)-infected gastric epithelial AGS cells. AGS cells were stimulated with H. pylori(NCTC 11637) at the ratio of 300:1(bacterium:cell). Western blot analysis revealed that 14-3-3 was elevated at 3 hr after H. pylori treatment. Other isoforms were not significantly affected by H. pylori infection. Using immunoprecipitation to 14-3-3 , followed by proteomic analysis, we found that S phase kinase associated protein isoform 2 bound to 14-3-3 has increased. In contrast, three proteins (DEAD-box polypeptide 3, heterogeneous nuclear ribonucleoprotein H2 and WD repeat-containing protein isoform 1) bound to 14-3-3 decreased by H. pylori infection. Our results suggest that 14-3-3 may play an important regulatory role in H. pylori-induced signal transduction in gastric epithelial cells.
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KEYWORD
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Helicobacter pylori, gastric epithelial cells, 14-3-3, 14-3-3 associated proteins, proteomics
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